Siab -kev ua haujlwm ua kua chromatography (HPLC) thiab roj chromatography (GC) feem ntau yog siv los txheeb xyuas.
Txhawm rau kom paub meej tias tus qauv tsis paub yog Kathon preservative thiab nws purity, qhov kev sim no tau siv cov txheej txheem kev suav vector hauv Millennium 2010 software los sib piv cov spectral sib txawv ntawm ob yam, 2-methyl-4-isothiazolin-3-one thiab 2-methyl-5-chloro-4-isothiazolin-3-one, hauv Kathon preservative. Qhov no suav nrog kev tshuaj xyuas lub ncov chromatographic ntawm tag nrho cov wavelength; uas yog, scanning ntawm txhua qhov taw tes hauv chromatogram (los yog txhua lub sijhawm) yields vector lub kaum sab xis hauv ultraviolet spectrum, uas ua rau lub kaum sab xis ntawm ntau qhov hu ua lub purity angle. Lub kaum sab xis purity qhia txog qhov sib txawv ntawm lub ncov chromatographic (piv txwv li, purity ntawm lub ncov). Lub purity lub kaum sab xis ntawm 0℃mus rau 90 degree; tus nqi qis dua qhia tias siab dua purity, thiab vice versa. Hauv daim duab purity matching, qhov kev sib tshuam ntawm purity nkhaus thiab chromatographic ncov qhia qhov sib txawv tseem ceeb ntawm lub spectrum ua ntej thiab tom qab ntawd lub sij hawm tuav thiab lub spectrum ntawm lub ncov ncov, qhia lub kaum sab xis loj dua thiab muaj peev xwm ntawm impurities. Lub purity lub kaum sab xis yog xam ntau npaum li cas rau txhua lub wavelength ntawm txhua qhov taw tes ntawm chromatographic ncov, yielding spectral sib txawv (qhov tseeb suav suav nrog cov qauv complex, tau ua tiav los ntawm Millennium 2101 chromatographic tswj system thiab 996 software). Qhov ntau yam spectra yog ces muab piv.
Raws li lub hauv paus ntsiab lus ntawm kev sib piv spectral thiab retrieval los ntawm cov txheej txheem tsim database, kev txiav txim siab thiab kev soj ntsuam zoo ntawm Kathon preservatives ua tau yooj yim thiab txhim khu kev qha.
